Syllabus

MPL 103T: Pharmacological and Toxicological Screening

Methods - I

Theory                                                                                                       (60 hours)

Unit-1                                                                                                  (12 hours)

1.    Laboratory Animals

        Common Laboratory Animals: Description, handling and applications of different species and strains of animals.

        Transgenic Animals: Production, maintenance and applications Anaesthesia and euthanasia of experimental animals.

        Maintenance and breeding of laboratory animals

        CPCSEA guidelines to conduct experiments on animals

        Good Laboratory Practice

        Bioassay: Principle, scope and limitations and methods

Unit-2                                                                                                 (12 hours)

2.     Preclinical Screening of New Substances for the Pharmacological Activity Using In-vivo, In-vitro, and other possible Animal Alternative Models.

        General Principles of Preclinical Screening. CNS Pharmacology: Behavioral and muscle coordination, CNS stimulants and depressants, anxiolytics, anti-psychotics, anti-epileptics and nootropics. Drugs for neurodegenerative diseases like Parkinsonism, Alzheimer’s and multiple sclerosis. Drugs acting on Autonomic Nervous System

Unit-3                                                                                                 (12 hours)

3.     Preclinical Screening of New Substances for the Pharmacological Activity Using In-vivo, In-vitro, and other possible Animal Alternative Models.

Respiratory Pharmacology: Anti-asthmatics, drugs for COPD and Anti-allergics. Reproductive Pharmacology: Aphrodisiacs and Anti-fertility agents, Analgesics, Anti-inflammatory and Anti-pyretic agents.

Gastrointestinal Drugs: Anti-ulcer, Anti-emetic, Anti-diarrheal and Laxatives.

Unit-4                                                                                                                                 (12 hours)

4.     Preclinical Screening of New Substances for the Pharmacological Activity Using In-vivo, In-vitro, and other possible Animal Alternative Models.

Cardiovascular Pharmacology: Anti-hypertensives, Anti-arrythmics, Anti-anginal, Anti-atherosclerotic agents and Diuretics. Drugs for metabolic disorders like Anti-diabetic, Anti-dyslipidemic agents.

Anti-cancer agents. Hepatoprotective screening methods

Unit-5                                                                                                                                  (12 hours)

5.     Preclinical Screening of New Substances for the Pharmacological Activity Using In-vivo, In-vitro, and other possible Animal Alternative Models.

Immunomodulators, Immunosuppressants and Immunostimulants

General Principles of Immunoassay: Theoretical basis and optimization of immunoassay, heterogeneous and homogenous immunoassay systems. Immunoassay methods evaluation; protocol outline, objectives and preparation. Immunoassay for digoxin and insulin

Limitations of animal experimentation and alternate animal experiments

Extrapolation of in-vitro data to preclinical and preclinical to humans